High-throughput profiling of tissue and tissue model microarrays: Combined transmitted light and 3-color fluorescence digital pathology
Michel Nederlof1, Shigeo Watanabe2, Bill Burnip3, D Lansing Taylor4, Rebecca Critchley-Thorne1
1 Cernostics, Inc., 1401 Forbes Avenue, Suite 302, Pittsburgh, PA 15219, USA
2 Hamamatsu Photonics, K.K., 812 Joko-Cho, Higashi-Ku, Hamamatsu City, 431-3196, Japan
3 Hamamatsu Corporation, 2593 Wexford-Bayne Rd, Suite 305, Sewickley, PA 15143, USA
4 University of Pittsburgh Drug Discovery Institute, Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, 15260, USA
Cernostics, Inc., 1401 Forbes Avenue, Suite 302, Pittsburgh, PA 15219
© 2011 Nederlof et al; This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
For many years pathologists have used Hematoxylin and Eosin (H&E), single marker immunohistochemistry (IHC) and in situ hybridization with manual analysis by microscopy or at best simple digital imaging. There is a growing trend to update pathology to a digital workflow to improve objectivity and productivity, as has been done in radiology. There is also a need for tissue-based multivariate biomarker assays to improve the accuracy of diagnostic, prognostic, and predictive testing. Multivariate tests are not compatible with the traditional single marker, manual analysis pathology methods but instead require a digital platform with brightfield and fluorescence imaging, quantitative image analysis, and informatics. Here we describe the use of the Hamamatsu NanoZoomer Digital Pathology slide scanner with HCImage software for combined brightfield and multiplexed fluorescence biomarker analysis and highlight its applications in biomarker research and pathology testing. This combined approach will be an important aid to pathologists in making critical diagnoses.